Recombinant:Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
Article Title: Microenvironmental acidosis drives PARP- and ATM inhibitor resistance in p53 deficient pancreatic cancer
Article Snippet: Cat#MA1-935, RRID:AB_2081230 RasG12D Cell Signaling Technology Cat#sc-14429, RRID:AB_2728748 p53 Cell Signaling Technology CS-2524S p150 BD Biosciences BD-610474, RRID:AB_397846 NHE1 (WB) Santa Cruz Biotechnology Sc-136239, RRID:AB_2191254 NBCn1 (WB) Abcam Ab82335, RRID:AB_10672662 MCT1 Santa Cruz Biotechnology Sc-365501, RRID:AB_2943297 MCT4 Santa Cruz Biotechnology sc-376140,RRID:AB_2943304 Slc26a6 Santa Cruz Biotechnology Sc-515230, RRID:AB_10977408 CFTR (WB) Thermo Fisher Scientific. .. MA1-935, RRID:AB_2081230 γ-H2AX Cell Signaling Technology Cat#CS-2577 PARP1 (total and cleaved) Cell Signaling Technology CatCS-9542 ATM Cell Signaling Technology Cat#CS-2873 p-ATM/ATR Cell Signaling Technology Cat#6966 β-actin Sigma Aldrich A5441 ATP6V1B1 Santa Cruz Biotechnology Cat# Sc-55544, RRID:AB_831844 AE2/Slc4a2 (WB) Antibodies.com Cat#A268836 AE3/Slc4a3 (WB) Origene Cat#TA371763 MCT8/Slc16a2 Proteintech Cat#20676-1-AP, RRID:AB_10695188 pCHK1 Cell Signaling Technology Cat#2328, RRID:AB_331212 Total CHK1 Cell Signaling Technology Cat#2360, RRID:AB_2080320 pATR Cell Signaling Technology Cat#30632, RRID:AB_2798992 Alexa-488 goat-antirabbit secondary antibody Thermo Fisher Scientific Cat#A11070, RRID:AB_2534114 Alexa Fluor-647 donkey-anti-mouse secondary antibody Thermo Fisher Scientific Cat#A31571, RRID:AB_162542 Goat Anti-Rabbit Immunoglobins HRP conjugated secondary antibody Dako Cat#P0448, RRID:AB_2617138 Goat Anti-Mouse Immunoglobins HRP conjugated secondary antibody Dako Cat#P0447, RRID:AB_2617137 NBCn1 inhibitory antibody Kindly gifted from E. Boedtkjer, Aarhus University – mCAIX/Car9 Abcam Cat#Ab15086, RRID:AB_2066533 Chemicals, peptides, and recombinant proteins CellEvent Caspase-3/7 Detection Reagent ThermoFisher Scientific Cat#C10423 Trypan Blue Sigma Aldrich Cat#T8154 IGEPAL CA-360 Sigma Aldrich Cat#I8896 (Continued on next page) e1 iScience 29, 115112, March 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER phosphatase inhibitor cocktail (PhosStop) cOmpleteTM Roche Cat#04906845001 Protease Inhibitor mix cOmpleteTM Roche Cat#1169748001 Dynabeads Protein G Invitrogen Cat#10004D NuPAGE LDS Sample Buffer Invitrogen #NP0007 24-well plate BioLite Multidish Thermo Fisher Scientific Cat#930186 Matrigel Growth Factor Reduced Corning Cat #356231 Advanced F-12 DMEM Thermo Fisher Scientific Cat#12634010 Corning Cell Recovery Solution Corning Cat#354253 DMSO Sigma Aldrich Cat#D2650 AZD0156 MedChemExpress HY-100016 Olaparib Nordic Biosite Cat#154-10621-5 Slide 8 Well chambered coverslips Ibidi Cat#80806 VWR Chemicals Cat#9713.1000 Dulbecco’s Phosphate-Buffered Saline containing Ca 2+ and Mg 2+ (DPBS +/+) Thermo Fisher Scientific Cat#14040133 bovine serum albumin (BSA) Sigma Aldrich A7906 Triton-X Sigma Aldrich X100 sodium azide Sigma Aldrich S2002-25g DAPI Invitrogen Cat#D3571 Fluoromount-G Invitrogen Cat#00-4988-02 SNARF-1 salt Thermo Fisher Scientific Cat#C1270 Lab-Teck II Glass Chamber Slides Thermo Fisher Scientific Cat#154534PK Nigericin Sigma Aldrich Cat# N7143-5mg Trypsin-EDTA Thermo Fisher Scientific Cat#T4174 96-well plates Corning Cat#3595 Olaparib Cayman Cat#10621 Cariporide MedChemExpress HY-19693 DIDS Merck A0554-250MG S0859 MedChemExpress HY-15529 AZD0095 Selleckchem E1549 Ponceau Sigma-Aldrich #P7170-1L BenchMark Protein Ladder Thermo Fisher Scientific #10747012 4% formaldehyde stabilised, buffered (pH 7.0+0.2) VWR Chemicals 9713.1000 Critical commercial assays IncuCyte® S5 Live-Cell Analysis System Essen BioScience Deposited data RNA-seq data GEO Database GSE218178 Experimental models: Cell lines Murine pancreatic organoids Gifted from the Tuveson lab: Boj et al. 33 – Software and algorithms Colab https://colab.google/ – Python https://www.python.org/ – R language https://www.r-project.org/ – Graphpad Prism v 10 https://www.graphpad.com/updates/ prism-1000-release-notes – (Continued on next page) iScience 29, 115112, March 20, 2026 e2
RNA Extraction:Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
Real-time Polymerase Chain Reaction:Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
BIA-KA:Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
Membrane:Article Title: STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degeneration.
Article Snippet: .. The following primary antibodies were used to probe the membrane; anti-CRYBA1 (1:1000, Invitrogen, Cat. #PA5-28954), anti-STING (1:1000, Novus Biologicals, Cat. # NBP2-24683), anti-IL17A (1:1000, Abcam, Cat. #ab79056), anti-IFNAR1 (1:1000, Proteintech, cat#83002-4-RR), anti-phospho STAT1 (1:1000, Abcam, cat#109461), STAT1 (1:1000, Cell Signaling Technology, Cat. #14994), anti-phospho STAT3 (Tyr705) (1:1000, Cell Signaling Technology, Cat. #9131), anti-STAT3 (1:1000, Cell Signaling Technology, Cat. #4904), anti-cleaved caspase 1 (1:1000, Cell Signaling Technology, Cat. #4199), anti-H3 (1:2000, Cell Signaling Technology, Cat. #4499, Cell Signaling Technology), anti--Actin (1:2000, Cat. #4970, Cell signaling technology) and anti-Vinculin (1:2000, Cell Signaling Technology, Cat. #13901). .. After primary incubation, membranes were washed three times in TBST for 10 minutes each and incubated with HRP-conjugated anti-rabbit IgG secondary antibody (1:3000, Cell Signaling Technology, Cat. #7074) for 1 h at RT with gentle shaking.
Article Title: STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degeneration
Article Snippet: .. The following primary antibodies were used to probe the membrane; anti-CRYBA1 (1:1000, Invitrogen, Cat. #PA5-28954), anti-STING (1:1000, Novus Biologicals, Cat. #NBP2-24683), anti-IL17A (1:1000, Abcam, Cat. #ab79056), anti-IFNAR1 (1:1000, Proteintech, Cat. #83002-4-RR), anti-phospho STAT1 (1:1000, Abcam, Cat. #109461), STAT1 (1:1000, Cell Signaling Technology, Cat. #14994), anti-phospho STAT3 (Tyr705) (1:1000, Cell Signaling Technology, Cat. #9131), anti-STAT3 (1:1000, Cell Signaling Technology, Cat. #4904), anti-cleaved caspase 1 (1:1000, Cell Signaling Technology, Cat. #4199), anti-H3 (1:2000, Cell Signaling Technology, Cat. #4499, Cell Signaling Technology), anti-β-Actin (1:2000, Cat. #4970, Cell Signaling Technology) and anti-Vinculin (1:2000, Cell Signaling Technology, Cat. #13901). .. After primary incubation, membranes were washed three times in TBST for 10 min each and incubated with HRP-conjugated anti-rabbit IgG secondary antibody (1:3000, Cell Signaling Technology, Cat. #7074) for 1 h at RT with gentle shaking.
Western Blot:Article Title: Microenvironmental acidosis drives PARP- and ATM inhibitor resistance in p53 deficient pancreatic cancer
Article Snippet: Cat#MA1-935, RRID:AB_2081230 RasG12D Cell Signaling Technology Cat#sc-14429, RRID:AB_2728748 p53 Cell Signaling Technology CS-2524S p150 BD Biosciences BD-610474, RRID:AB_397846 NHE1 (WB) Santa Cruz Biotechnology Sc-136239, RRID:AB_2191254 NBCn1 (WB) Abcam Ab82335, RRID:AB_10672662 MCT1 Santa Cruz Biotechnology Sc-365501, RRID:AB_2943297 MCT4 Santa Cruz Biotechnology sc-376140,RRID:AB_2943304 Slc26a6 Santa Cruz Biotechnology Sc-515230, RRID:AB_10977408 CFTR (WB) Thermo Fisher Scientific. .. MA1-935, RRID:AB_2081230 γ-H2AX Cell Signaling Technology Cat#CS-2577 PARP1 (total and cleaved) Cell Signaling Technology CatCS-9542 ATM Cell Signaling Technology Cat#CS-2873 p-ATM/ATR Cell Signaling Technology Cat#6966 β-actin Sigma Aldrich A5441 ATP6V1B1 Santa Cruz Biotechnology Cat# Sc-55544, RRID:AB_831844 AE2/Slc4a2 (WB) Antibodies.com Cat#A268836 AE3/Slc4a3 (WB) Origene Cat#TA371763 MCT8/Slc16a2 Proteintech Cat#20676-1-AP, RRID:AB_10695188 pCHK1 Cell Signaling Technology Cat#2328, RRID:AB_331212 Total CHK1 Cell Signaling Technology Cat#2360, RRID:AB_2080320 pATR Cell Signaling Technology Cat#30632, RRID:AB_2798992 Alexa-488 goat-antirabbit secondary antibody Thermo Fisher Scientific Cat#A11070, RRID:AB_2534114 Alexa Fluor-647 donkey-anti-mouse secondary antibody Thermo Fisher Scientific Cat#A31571, RRID:AB_162542 Goat Anti-Rabbit Immunoglobins HRP conjugated secondary antibody Dako Cat#P0448, RRID:AB_2617138 Goat Anti-Mouse Immunoglobins HRP conjugated secondary antibody Dako Cat#P0447, RRID:AB_2617137 NBCn1 inhibitory antibody Kindly gifted from E. Boedtkjer, Aarhus University – mCAIX/Car9 Abcam Cat#Ab15086, RRID:AB_2066533 Chemicals, peptides, and recombinant proteins CellEvent Caspase-3/7 Detection Reagent ThermoFisher Scientific Cat#C10423 Trypan Blue Sigma Aldrich Cat#T8154 IGEPAL CA-360 Sigma Aldrich Cat#I8896 (Continued on next page) e1 iScience 29, 115112, March 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER phosphatase inhibitor cocktail (PhosStop) cOmpleteTM Roche Cat#04906845001 Protease Inhibitor mix cOmpleteTM Roche Cat#1169748001 Dynabeads Protein G Invitrogen Cat#10004D NuPAGE LDS Sample Buffer Invitrogen #NP0007 24-well plate BioLite Multidish Thermo Fisher Scientific Cat#930186 Matrigel Growth Factor Reduced Corning Cat #356231 Advanced F-12 DMEM Thermo Fisher Scientific Cat#12634010 Corning Cell Recovery Solution Corning Cat#354253 DMSO Sigma Aldrich Cat#D2650 AZD0156 MedChemExpress HY-100016 Olaparib Nordic Biosite Cat#154-10621-5 Slide 8 Well chambered coverslips Ibidi Cat#80806 VWR Chemicals Cat#9713.1000 Dulbecco’s Phosphate-Buffered Saline containing Ca 2+ and Mg 2+ (DPBS +/+) Thermo Fisher Scientific Cat#14040133 bovine serum albumin (BSA) Sigma Aldrich A7906 Triton-X Sigma Aldrich X100 sodium azide Sigma Aldrich S2002-25g DAPI Invitrogen Cat#D3571 Fluoromount-G Invitrogen Cat#00-4988-02 SNARF-1 salt Thermo Fisher Scientific Cat#C1270 Lab-Teck II Glass Chamber Slides Thermo Fisher Scientific Cat#154534PK Nigericin Sigma Aldrich Cat# N7143-5mg Trypsin-EDTA Thermo Fisher Scientific Cat#T4174 96-well plates Corning Cat#3595 Olaparib Cayman Cat#10621 Cariporide MedChemExpress HY-19693 DIDS Merck A0554-250MG S0859 MedChemExpress HY-15529 AZD0095 Selleckchem E1549 Ponceau Sigma-Aldrich #P7170-1L BenchMark Protein Ladder Thermo Fisher Scientific #10747012 4% formaldehyde stabilised, buffered (pH 7.0+0.2) VWR Chemicals 9713.1000 Critical commercial assays IncuCyte® S5 Live-Cell Analysis System Essen BioScience Deposited data RNA-seq data GEO Database GSE218178 Experimental models: Cell lines Murine pancreatic organoids Gifted from the Tuveson lab: Boj et al. 33 – Software and algorithms Colab https://colab.google/ – Python https://www.python.org/ – R language https://www.r-project.org/ – Graphpad Prism v 10 https://www.graphpad.com/updates/ prism-1000-release-notes – (Continued on next page) iScience 29, 115112, March 20, 2026 e2
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